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Rex - the titular character of the series is a Amnesiac Evo (apparently a slang variation of the word Evolution) like all of the "Evo's" he has suffered a unique mutation, his being the ability to grow machines out of his body for virtually any occasion and then absorb them back into himself when done (this power is not unlimited he can only grow certain machines out of specific parts of his body). He can also cure some Evo's of their Mutations in addition to using his Nanites to communicate and control nearby machines. Agent Six believes that there is something wrong with him (aside from the amnesia and Nanites) and believes that the reason why he's struggling with his nanites is because of his inability to control his emotions. Rex often vents his frustrations (such as the size of his room and the "all work and no play" outlook of Agent Six) by riding around the Evo habitat on his motor bike and breaking out of the base to go on "Road Trips" with Bobo Haha.
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Bobo Haha - an Evo (as apparent by his ability to talk) monkey who is Rex's sidekick. He wears a eye patch and fez. At one point he stated that he's a bad influence as well as the fact that no one listens to him. Aside from the ability to talk he seems to be a completely normal monkey. In the concept art he's shown using a pair of laser pistols and the same goes for the series. He is voiced by John DiMaggio.
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Agent Six - an agent of "Providence", a secret organization dedicated to capturing and deactivating the Evo's. He wears a green suit with a black tie and black sunglasses. Due to his strict by-the-book personality it's made clear that he only tolerates Rex and by extension any Evo on the side of good because they help stop the aggressive or dangerous Evo's (Rex goes as far as to refer to him as a "Nanny just more Agro"). Rex at one point commented that he knows several forms of martial arts and hopes he will teach Rex some of them. In the concept art he's shown using a pair of high-tech beyonettes however in the series he uses a pair of fold up Katanna. He is voiced by Wally Kurth.
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Dr. Holiday - (name confirmed on Cartoon Network) a scientist for "Providence", a secret organization dedicated to capturing and deactivating Evo's. She is usually seen wearing a lab coat, an orange top, and knee-high boots. She is depicted as the polar opposite of Agent Six, as she is much more in-tune with her emotions, in response to Agent Six's stoic nature. She is the only one who sees Rex as more than a machine. She repeatedly tries to convice Six to try to relate to Rex, as she understands what he goes through with his amnesia and the pressure put on him by Providence. She may have a crush on agent Six as she tried to hold his hand but as of yet has been rejected due to his stoic nature.
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White Knight (the leader of Providence) is very determined to stop the Evo's. He is scared of coming into contact with the Evo's or the the Nanites (which are so wide spread they are in every living thing on the planet but most are dormant and harmless). As far as anyone can tell he never leaves the sterilized room he calls an office, as apparent by his flour white skin and that he keeps a jar of milk in a drawer.
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Van Kleiss - Van Kleiss is described as every mad scientist you ever saw only a lot freakier and dangerous. His body's molecular structure is unstable and thus has to stabilize himself using Nanites extracted from Evo's Captured by the Pack and experimented upon by him. He possesses the ability to control everything in his Kingdom Abysus but out of his territory he cannot use his powers. He knows a great deal about the Nanites which causes Evo Mutations and about Rex's past (implying he had a hand in his mutation) he states that he will tell Rex everything but only if Rex joins his team. He is voiced by Troy Baker
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Breach - Breach can open portals from anywhere to anywhere else making her the Packs form of Transportation as well as fire the portals at her enemies like Shurikun and send attacks through her portals making her the Packs' most unpredictable member.
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Biowulf - A armored werewolf like Evo with super human abilities and is fiercely loyal to Van Kleiss. Due to Van Kleiss powers being limited to his native soil Biowulf acts like the Pack's Defacto leader.
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Skalamander - A humanoid Lizard Evo with crystal spikes growing out of him and a crystal club growing out of his left arm. He can fire said spikes out of the club or grow it into a sheild providing short or long range attacks and defensive abilities as well.